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Proteomics is a a large-scale and comprehensive study of proteins, which are vital parts of any living organism’s functioning, including protein abundances, variations and modifications. In the discovery of proteomics, the bottom-up approach and top-down approach are used for pretome analysis. In bottom-up method, a crude mixture is subjected to protease digestion, then liquid chromatography separation is performed, followed by MS analysis, while MS characterization of protein without prior proteolysis is done under the top-down method, providing greater sequence coverage, prevent sequence ambiguities and PTMs preservation.
There are different services offered under proteomics which include protein identification (database searching, peptide mass fingerprint, accurate mass and time tag, de novo sequencing, etc.), protein quantification (chemical protein labeling, enzymatic peptide labeling, metabolic labeling, etc.), sample extraction (biopsy, biofluid, primary cell culture, laser capture microdissection, etc.), protein fractionation (isoelectric focusing, cell surface labeling, affinity depletion, etc.), peptide fractionation (strong cation exchange, ion-pairing reversed phase, weak anion exchange, etc.), and mass spectometry (electrospray ionization, matrix assisted laser desorption, ion trap MS, etc.). Quantification of sample protein relative to another protein sample of in absolute terms are the major tasks in most protein profiling projects. Data-dependent methods and data-independent methods are the techniques now used in protein profiling. Data-dependent method involves choice of peptide fragments for subsequent MS/MS analysis basing on pre-determined criteria, whereas data-independent methods involves fragmentation of all peptides indiscriminately, with a pre-established mass range. Although data-independent methods are relatively new, there are promising results for the future of quantitative proteomics, because it is potentially able to analyze all species present in a specific sample at concentrations which are detectable, using a single assay, and the data can be used as improved software, as well as silico databases become available.
Proteomics research is used in many scientific fields such as cancer research, diabetes research, discovery of diagnostic biomaker, plant and animal phenotyping, microbiology, drug discovery and drug development, toxicological testing, and study and treatment of disease through antibody profiling. A proteomics company employs highly knowledgeable and experienced biological scientists, providing proteomic services including protein gel and imaging analysis, protein identification, protein quantification, protein-protein interaction, top-down proteomics, peptidomics service, protein post-translational modification analysis, and many more. With the advancement of technology, mass spectometry is utilized for protein and peptide identification, shortening time duration, thus enhancing the accuracy of protein sequencing.
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A respected and well-known proteomics company offers proteomics services to assist you in our scientific research, including molecular weight determination, molecular weight distribution of peptides and proteins service, sample preparation, digestion service, peptides or protein purity analysis, peptide mass fingerprinting (PMF), peptides and proteins sequence analysis, de novo protein sequence analysis, shotgun protein identification, accurate mass determination, membrane proteomics and subcellular proteomics service.5 Key Takeaways on the Road to Dominating Science